Development of Novel Rice Germplasm for Salt-Tolerance at Seedling Stage Using CRISPR-Cas9

Saline-alkali tolerant rice, as the first selected grain crop for improving coastal tidal land and saline-alkali land, has great potential comprehensive utilization. In this study, an elite three-line restorer in breeding, R192, was taken receptor, CRISPR/Cas9 technology used to perform directional editing of OsRR22, main effect gene, which controls salt tolerance rice. Eight transgenic plants T0 generation with OsRR22 gene knockout were obtained, seedlings screened by using PCR amplification sequence comparison. The homozygous mutant lines, M16 M18, knockout, did not contain a vector skeleton, identified T2 generation. There +1 bp −20 exon regions respectively. At three-leaf one-heart stage, treated 0.4% 0.8% NaCl solution, then their during seedling stage identified. results showed that, without treatment, no significant differences found plant height, number green leaves, total dry weight, fresh weight between lines M18 wildtype (WT) R192. However, after treatment two different concentrations, mutants extremely comparison WT weight; WT, there treatments. Our indicate new germplasm mutation induced could improve providing reference improvement